| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Brevibacillus massiliensis | GCAAGGCGTTAGAGATTGAGG | TCGGCTCTTTCTTCCCAGG | 59.06 | 59.02 | 178 | 127 |
100.00%
|
100.00%
|
| Primer 2 | Brevibacillus massiliensis | GCAAGGCGTTAGAGATTGAGG | TTCGGCTCTTTCTTCCCAGG | 59.06 | 59.68 | 179 | 127 |
100.00%
|
100.00%
|
| Primer 3 | Brevibacillus massiliensis | GGCGTTAGAGATTGAGGAAGG | TCGGCTCTTTCTTCCCAGG | 58.17 | 59.02 | 174 | 118 |
100.00%
|
100.00%
|
| Primer 4 | Brevibacillus massiliensis | GGCGTTAGAGATTGAGGAAGG | TTCGGCTCTTTCTTCCCAGG | 58.17 | 59.68 | 175 | 118 |
100.00%
|
100.00%
|
| Primer 5 | Brevibacillus massiliensis | TGCTCTGTGACCAGCAAGG | ACCGACATTCCGCTGTACC | 59.93 | 59.78 | 293 | 105 |
100.00%
|
100.00%
|
| Primer 6 | Brevibacillus massiliensis | AGTGGAAGGCATGTCCTGC | AAACGACGGTGTACCCCTG | 60.00 | 59.63 | 179 | 104 |
100.00%
|
100.00%
|
| Primer 7 | Brevibacillus massiliensis | CCGAACACTCCGCAAAAGC | GCAGCCAAGTAAGCATCTGG | 60.08 | 59.26 | 265 | 103 |
100.00%
|
100.00%
|
| Primer 8 | Brevibacillus massiliensis | TCAGGTTCCGGTTGCTCTG | AGTGGTACGGGTTTGGAAGG | 59.63 | 59.60 | 159 | 103 |
100.00%
|
100.00%
|
| Primer 9 | Brevibacillus massiliensis | TGCAAAAGGATTGGCGTGC | CCCCATTTTCCTCTTCGTGC | 60.01 | 59.19 | 176 | 100 |
100.00%
|
100.00%
|
| Primer 10 | Brevibacillus massiliensis | ATCTGGAGCGGTCTCTTCC | TCAGCCAATTCCGTCGACC | 58.49 | 60.08 | 199 | 100 |
100.00%
|
100.00%
|